Goat, Goat anti-biotin, TRITC conjugated

Name

Goat, Goat anti-biotin, TRITC conjugated

Size

1 mg

Catalog number

AS10 1455

Price

312 EUR

More details

Available ordering format

Liquid

Immunogen

Biotin

Raised in

Goat

Clonality

Polyclonal

Clone

Polyclonal

Purification

Affinity purified IgG

How to reconstitute

See included datasheet or contact our support service

Storage condition

2-8°C. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.

Verified applications

Contact Gentaur support center

Connected products

other secondary antibodies against biotin

Recommended dilutions for use

This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2000 for most applications.

Molecular weight (expected | аpparent)

see datasheet

Verified reactivity

to be determined

Possible reactivity

to be determined

No reactivity

to be determined

Supplementary information

Contact Gentaur support center at info@gentaur.com

References

Refer to PubMed

Scientific context

Goat anti-biotin is a secondary antibody conjugated to TRITC, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin. Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm

Notes

Purity of this preparation is> 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.

Protein number

Refer to NCBI

TAIR number

Refer to NCBI

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Conjugation

Biotinylated

Latin name

Capra aegagrus hircus