Goat, Goat anti-biotin, TRITC conjugated
1 mg
AS10 1455
312 EUR
Liquid
Biotin
Goat
Polyclonal
Polyclonal
Affinity purified IgG
See included datasheet or contact our support service
2-8°C. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Contact Gentaur support center
other secondary antibodies against biotin
This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2000 for most applications.
see datasheet
to be determined
to be determined
to be determined
Contact Gentaur support center at [email protected]
Refer to PubMed
Goat anti-biotin is a secondary antibody conjugated to TRITC, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin. Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.
Refer to NCBI
Refer to NCBI
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Biotinylated
Capra aegagrus hircus