Goat Polyclonal Serum Anti-RAGE, human, Receptor for Advanced Glycation End Products

Name

Goat Polyclonal Serum Anti-RAGE, human, Receptor for Advanced Glycation End Products

Size

100 µl

Catalog number

RAGE001

Price

494 EUR

More details

Availability

Please contact us to check the availability

Immunogen

Synthetic peptide corresponding to human RAGE (42-59)

Specificity

Human Receptor for Advanced Glycation End Products

Species reactivity

Human, cross-reactivity with mouse, rat and bovine receptor expected

Product presentation

Serum containing 0.01% Merthiolate (Thimerosal)

Product category

Primary Antibody

Antibody's raised in

Goat

Antibody's isotype

Serum

Antibody's clone

polyclonal

Product is supplied as

liquid concentrate

Recommended concentration for use

IHC (P) (1:400), ELISA (1:10000-1:260000), WB 1:2000

Protein concentration

N/A

Positive control

human renal tissue

Pretreatment

In immunohistochemistry of formaldehyde-fixed paraffin embedded tissue we recommend high temperature antigen unmasking with citrate buffer (Art. No. DE000) 15-30 min.

Tested applications

IHC(P), ELISA , WB

Recommended secondary reagent

We recommend the use of anti-goat IgG:Biotin (Art. No. BA-9500) in combination with Streptavidin:HRPO (Art. No. SA-5004) and a suitable chromogenic substrate e.g. DAB (Art. No. DA002) or AEC (Art. No. AE002).

Storage method

Store at -20°C

UniProt number

Q151Go to UniProt/SwissProt9 (RAGE_HUMAN)

Litterature

1. Schmidt, A.M., Hori O., Cao R., Yan S.D., Brett J., Wautier J.L., Ogawa S., Kuwabara K., Matsumoto M., and Stern D. (1996) RAGE: a novel cellular receptor for advanced glycation end products. Diabetes 45 Suppl. 3; S 77-80 Review.

Tips

Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** Merthiolate (Thimerosal) is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low Merthiolate concentration it must be handled with care. Dispose according to regional rules!

Tissue

serum

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.

Latin name

Capra aegagrus hircus

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Group

Polyclonals and antibodies,Sera

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Serum should be stored at +5°C