Anti-Goat IgG (H+L), Human SP ads-FITC

Name

Anti-Goat IgG (H+L), Human SP ads-FITC

Size

1000ug

Catalog number

GEN675019

Price

227 EUR

More details

Also known as

Rabbit Anti-Goat IgG (H+L), Human SP ads

Other names

N/A

Category

Secondary Antibodies

Subcategory

Mnoclonal antibodies

Gene name

N/A

Gene name synonims

N/A

Other gene names

N/A

Clonality

Monoclonal

Immunoglobulin isotype

IgG

Clone

N/A

Host organism

Rabbit (Oryctolagus cuniculus)

Species reactivity

Goat; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Specificity and cross-reactivity

Reacts with the heavy and light chains of goat IgG as demonstrated by ELISA. May also react with the light chains of other goat immunoglobulins. Minimal cross reactivity with human serum proteins.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Purification method

Affinity chromatography on goat IgG covalently coupled to agarose.

Form/Appearance

Fluorescein (FITC) Conjugate

Concentration

N/A

Storage and shipping

The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius..

Tested applications:

Immunofluoresent staining, ELISA, Western Blot, Dot- and slot-immunoblotting, Immunohistochemistry

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG