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anti-Goat Anti-Mouse IgG (H&L) Biotin

Name

anti-Goat Anti-Mouse IgG (H&L) Biotin

Size

Goat Anti-Mouse IgG (H&L) Biotin

Catalog number

6918-100

Price

262 EUR

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More details

Target of this antibody:

Mouse IgG

Alternative names of antibody target:

Anti-mouse IgG, Biotin-Linked Antibody, Biotin linked secondary antibody

Background information:

Goat Polyclonal Secondary Antibody to mouse IgG (H&L) Biotin.

Antibody host:

Goat

Clonality of antibody:

Secondary antibody

Isotype:

Mouse IgG

Clone:

N/A

This antibody reacts with:

Goat

Immunogene sequence:

Mouse IgG (H&L)

Concentration of antibody:

N/A

Form of antibody:

Liquid

Formulation of this antibody:

In 0.01M Sodium phosphate, 0.25 M NaCl, 1% BSA, 50% Glycerol, pH 7.6 and 0.05% Sodium Azide.

Purification method:

Affinity Purified

This antibody has been succesfully tested for use in following methods:

ELISA, WB, IH, IC.

Recommended usage:

ELISA: 1:20,000 – 1:400,000, WB: 1:20,000 – 1:400,000, IH: 1:500 – 1:5000, IC: 1:500 – 1:5000.

Storage information:

Shelf time: 6 months in temp.: -20 deg.C

Shipping conditions:

gel pack

How to handle this antibody:

The antibody solution should be gently mixed before use.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Conjugation

Biotinylated

Latin name

Capra aegagrus hircus,Mus musculus

Additional isotype

Heavy & Light chains,IgG

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.