Goat Anti-Rat IgG Antibody (H+L), Biotin Conjugated

Name

Goat Anti-Rat IgG Antibody (H+L), Biotin Conjugated

Size

0.2ml

Catalog number

bs-0293G-Biotin

Price

121 EUR

More details

Category

Conjugated Secondary Antibody

Conjugation

Biotin

Host Organism

Goat

Target antigen

Rat IgG

Modification

Unmodified

Modification site

None

Clonality

Polyclonal antibody

Clone

Polyclonal antibody

Concentration

1ug/ul

Tested applications

WB, ELISA, IHC-P, IHC-F

Recommended dilutions

WB(1:500-2000), ELISA(1:500-2000), IHC-P(1:500-2000), IHC-F(1:500-2000)

Cross reactivity

Rat

Background of the target antigen

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Purification method

This antibody has been purified via Protein A.

Storage conditions

Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to one year.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Additional conjugation

Biotinylated

Latin name

Capra aegagrus hircus,Rattus norvegicus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Additional isotype

IgG

French translation

anticorps