Rabbit Anti-Goat IgM Antibody (H+L), Biotin Conjugated

Name

Rabbit Anti-Goat IgM Antibody (H+L), Biotin Conjugated

Size

0.2ml

Catalog number

bs-0370R-Biotin

Price

121 EUR

More details

Category

Conjugated Secondary Antibody

Conjugation

Biotin

Host Organism

Rabbit

Target antigen

Goat IgM

Modification

Unmodified

Modification site

None

Clonality

Polyclonal antibody

Clone

Polyclonal antibody

Concentration

1ug/ul

Tested applications

WB, ELISA, IHC-P, IHC-F

Recommended dilutions

WB(1:500-2000), ELISA(1:500-2000), IHC-P(1:500-2000), IHC-F(1:500-2000)

Cross reactivity

Goat

Background of the target antigen

Immunoglobulin IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells.

Purification method

This antibody has been purified via Protein A.

Storage conditions

Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to one year.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Additional conjugation

Biotinylated

Latin name

Capra aegagrus hircus,Oryctolagus cuniculus

Additional isotype

IgM

About

Rabbits are used for polyclonal antibody production by Bioss Secondary Antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. Bioss Secondary Antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

French translation

anticorps