Goat anti Rabbit IgA (Fc specific), conjugated with Biotin, Secondary Antibodies

Name

Goat anti Rabbit IgA (Fc specific), conjugated with Biotin, Secondary Antibodies

Size

1ml

Catalog number

GAR-IgA(Fc)-Bio

Price

612 EUR

More details

Host/Source

Goat

Clone Name

Polyclonal

Product Category

Veterinary

Species Reactivity

Rabbit

Application

ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting

Immunogen

Purified polyclonal IgA isolated from pooled rabbit serum. FreundÍs complete adjuvant is used in the first step of the immunization procedure.

Purification Method

NA

Shipping Conditions

NA

Storage conditions

Goat anti Rabbit IgA (Fc specific), conjugated with Biotin, Secondary Antibodies philized conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days

Datasheet link

NA

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs.

Conjugation

Biotinylated

Latin name

Capra aegagrus hircus,Oryctolagus cuniculus

Additional isotype

IgA

About

Rabbits are used for polyclonal antibody production by Exalpha. Rabbit antibodies are very stable and can be stored for several days at room temperature. Exalpha adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.