Goat anti Rat IgG2c (subclass specific), conjugated with Biotin, Secondary Antibodies

Name

Goat anti Rat IgG2c (subclass specific), conjugated with Biotin, Secondary Antibodies

Size

1ml

Catalog number

GARa-IgG2c-Bio

Price

612 EUR

More details

Host/Source

Goat

Clone Name

Polyclonal

Product Category

Veterinary

Species Reactivity

Rat

Application

ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting

Immunogen

Pools of purified homogenous IgG2c isolated from rat serum. FreundÍs complete adjuvant is used in the first step of the immunization procedure.

Purification Method

NA

Shipping Conditions

NA

Storage conditions

Goat anti Rat IgG2c (subclass specific), conjugated with Biotin, Secondary Antibodies philized conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days

Datasheet link

NA

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Conjugation

Biotinylated

Latin name

Capra aegagrus hircus,Rattus norvegicus

Additional isotype

IgG2c

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.