Goat anti Guinea Pig IgM (Fc specific), conjugated with FITC, Secondary Antibodies

Name

Goat anti Guinea Pig IgM (Fc specific), conjugated with FITC, Secondary Antibodies

Size

1ml

Catalog number

GAGp-IgM(Fc)-FITC

Price

487 EUR

More details

Host/Source

Goat

Clone Name

Polyclonal

Product Category

Veterinary

Species Reactivity

Guinea Pig

Application

ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence

Immunogen

Purified IgM isolated from guinea pig serum. FreundÍs complete adjuvant is used in the first step of the immunization procedure.

Purification Method

NA

Shipping Conditions

NA

Storage conditions

Goat anti Guinea Pig IgM (Fc specific), conjugated with FITC, Secondary Antibodies philized conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days

Datasheet link

NA

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular.

Properties

This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus

Additional isotype

IgM

About

Pigs and the smaller guinea pigs are frequent used as models for humans.