Amplite Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Name

Amplite Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Size

1000 Tests

Catalog number

11540

Price

345 EUR

More details

Description

Amplite Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence* is a kit that provides an optimized assay protocol that is compatible with HTS liquid handling, as little as 10 pg of a monoclonal antibody/well of a microplate can be detected.It can be used for assays that detect goat anti-mouse IgG as the secondary detection agent.

Shipping

Blue Ice

Storage

Freezer

Shelflife

6 months

Chemical Components

Component A: Amplite™ Red Peroxidase Substrate ; Component B: H2O2 ; Component C: Assay Buffer; Component D: DMSO; Component E: Goat Anti-Mouse IgG-HRP Conjugate

Excitation Wavelength

571 nm

Emission Wavelength

585 nm

Personal precautions

Use appropriate protective equipment and methods to clean up spilled substances promptly.

Uses

Laboratory chemicals *For Research Use Only*

Ordering

To order Amplite Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*, please use the Cat. Nr.11540 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information

Tips

Our specialists recommend you to follow carefully the pre-registered instructions for Amplite Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Additional description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

aat supplies other types of Assays as 1.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

Latin name

Capra aegagrus hircus,Mus musculus

Conjugation

HRP Conjugated,red dye

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG