Goat Anti-Rabbit IgG(H+L) FITC-conjugated

Name

Goat Anti-Rabbit IgG(H+L) FITC-conjugated

Size

50µL

Catalog number

S0008

Price

400 EUR

More details

Type

secondary antibody

Shipping

Ice bag

Antigen

Goat Anti-Rabbit IgG(H+L) FITC-conjugated

Reactivity

Rabbit

Clonality

Polyclonal

Application

WB,IF/ICC,FCM

Application_advice

IF 1:100 – 1:500

Conjugate

FITC

Immunogen

The antiserum was developed in goat using the Rabbit IgG as the immunogen.

Purification

Affinity-chromatography.

Format

Liquid

Concentration

1mg/ml

Fragment

Fab fragment

Storage_Buffer

0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6._x000D_ Store at -20℃,avoid repeated thawing and freezing.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

This Affinity Biosciences Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Affinity Biosciences FITC is highly sensitive to pH extremes.

Conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus,Oryctolagus cuniculus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by Affinity Biosciences. Rabbit antibodies are very stable and can be stored for several days at room temperature. Affinity Biosciences adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG