Swine anti Goat IgM (Fc specific)

Name

Swine anti Goat IgM (Fc specific)

Size

1 mL

Catalog number

SwAG/IgM(Fc)

Price

209 EUR

More details

Category

Secondary Antibodies

Long description

The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal goat serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in goat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of goat IgM from serum or plasma.

Antibody come from

Highly purified normal IgM isolated from pooled goat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Other description

Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal swine serum. No foreign proteins added.

Clone

Polyclonal

Antigen-antibody binding interaction

Swine anti Goat IgM (Fc specific) Antibody

Antibody is raised in

Swine

Antibody's reacts with

Caprine

Antibody's reacts with these species

The antiSerum does not cross-react with any other component of the Goat Ig system. Inter-species cross reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion a strong reaction with Sheep has been observed.

Antibody's specificity

Precipitating polyclonal Rabbit antiSerum to Goat IgM, Fc specific.

Research interest

Veterinary

Application

Immunoprecipitation

Antibody's suited for

Immunoprecipitation. In immunoelectrophoresis use 2 μl serum, plasma or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion and electroimmunodiffusion use 0.5 to 1.0 percent antiserum in the agar gel. Precipitin titre 1:32 when tested against pooled normal goat serum in agar-block immunodiffusion titration.

Storage

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days.

Relevant references

no information yet

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Latin name

Capra aegagrus hircus

Properties

Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Additional isotype

IgM