Goat anti Human IgE (Fc specific)
10 mg
SR/GAHu/IgE(Fc)/7S
348 EUR
Secondary Antibodies
The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and double radial immunodiffusion, using various erum concentrations against appropriate concentrations of IgE, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen. It does not react with human IgG, including all subclasses, IgA, IgM and IgG/Fab or any non-Ig protein in human serum. As unlabelled secondary antibody for indirect detection of IgE in human cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in human serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:7,500.
Purified homogenous IgE isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Polyclonal
Goat anti Human IgE (Fc specific) Antibody
Goat
Human
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail because of the low level of IgE in most species.
Specificity Reference Reagent. Purified IgG fraction of polyclonal Goat antiSerum to Human IgE, Fc specific.
Immunology
Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting
Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days.
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This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
Capra aegagrus hircus
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.Immunoglobulin E (IgE) kappa or Fc specific antibody is a kind of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is synthesized by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgEs play a role in allergy and response to parasite infection. High levels of IgEs caused by parasites can lower the allergic reaction of patients to allergens common present in the human environment. As such the parasite history of an allergic patient needs to be taken in consideration as a positive factor.
IgE