Goat anti Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains) antibody conjugated with TRITC
2 mL
GARa/IGg(H+L)/TRITC
400 EUR
Secondary Antibodies
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It is reacting with all known subclasses of rat IgG. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two Nordic immunoconjugates with different labels but originating from the same host animal species are used for direct two-color staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
Purified IgG isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Polyclonal
Goat anti Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains) antibody conjugated with TRITC Antibody
Goat
Rat
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this product has not been tested in detail.
Tetramethylrhodamine isothiocyanate–conjugated IgG fraction of polyclonal Goat antiSerum to Rat IgG, heavy and light chains
Veterinary
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
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see ncbi
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Capra aegagrus hircus,Rattus norvegicus
IgG1,IgG2a,IgG2b,IgG2c
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
anticorps