Goat anti Monkey lactoferrin antibody conjugated with FITC
1 mL
GAMon/Lfr/FITC
400 EUR
Primary Antibodies
In immunoelectrophoresis against rhesus monkey milk a single precipitin line is obtained. The antiserum does not react with any other protein component of monkey serum or plasma. As reagent for the direct detection of lactoferrin in monkey cells, tissues and body fluids in immunofluorescence; as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight antimicrobial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from monkey milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
Polyclonal
Goat anti Monkey lactoferrin antibody conjugated with FITC Antibody
Goat
Monkey
The antiSerum does not cross-react with any other Monkey plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however a strong cross-reaction with lactoferrin in Human milk has been observed.
Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Monkey lactoferrin.
Veterinary
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
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see ncbi
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Rhesus Monkeys proteins are often measured by ELISA on serum or plasma since consensus epitopes with the human ELISA are used for producing the antibodies of these ELISA test kits. Often cDNA of monkeys is used as alternative to human cDNA as a model for drug development. Monkeys are generally considered to be intelligent, particularly Old World monkeys.
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
Anti-FITC Antibody
Capra aegagrus hircus
anticorps