Goat anti Human J chain of dimeric IgA antibody conjugated with Biotin
1 mL
GAHu/J/Bio
400 EUR
Secondary Antibodies
In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), this immunoconjugate shows a single precipitation reaction with totally reduced and alkylated polyclonal and monoclonal polymeric IgA, secretory IgA and IgM. A reaction of complete identity is obtained with precipitated highly purified J chain and with the single of precipitation obtained with normal human serum after two hours incubation with 9M urea and 0.2M mercaptoethanol at pH 8.6. In a competitive radioimmunoassay no inhibition is obtained with monomeric IgA, polyclonal IgG, reduced and alkylated alpha, mu, gamma, kappa and lambda light chains (less than 1 percent over background). In immunocytochemical and immunohistochemical techniques for the detection of human J chain at the cellular and subcellular level in appropriately treated cell and tissue substrates; as detection reagent in nonisotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:200 and 1:10,000.
Human J chain is a polypeptide folded within the structure of the polymeric immunoglobulin, resulting in a very limited exposure of J chain antigenic determinants. The antiserum is raised against the isolated and purified J chain. J chains isolated from polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity; Human J chain is distinct from all other chain components of polymeric IgA and IgM. It has a unique primary structure as shown by sequence analyses. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
Polyclonal
Goat anti Human J chain of dimeric IgA antibody conjugated with Biotin Antibody
Goat
Human
The antiSerum does not cross-react with any other component of Human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. J chains of many related specie (e.g. Mouse, Rabbit, Dog Cat Sheep and Swine) are known to posses a striking structural homology. Cross-reactivity of this antiSerum has not been tested in detail.
Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Human J chain of dimeric IgA
Immunology
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
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see ncbi
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs.
Biotinylated
Capra aegagrus hircus
IgA
anticorps