Goat anti Human IgM (Fc specific) antibody conjugated with Horseradish peroxidase

Name

Goat anti Human IgM (Fc specific) antibody conjugated with Horseradish peroxidase

Size

1 mL

Catalog number

GAHu/IgM(Fc)/PO

Price

306 EUR

More details

Category

Secondary Antibodies

Long description

The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of human origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:8,000.

Antibody come from

Purified normal IgM and pools of homogenous IgM isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Other description

Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Clone

Polyclonal

Antigen-antibody binding interaction

Goat anti Human IgM (Fc specific) antibody conjugated with Horseradish peroxidase Antibody

Antibody is raised in

Goat

Antibody's reacts with

Human

Antibody's reacts with these species

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however a reaction has been obtained with Rhesus Monkey, Cynomolgus and Baboon.

Antibody's specificity

Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Human IgM, Fc specific

Research interest

Immunology

Application

ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting

Antibody's suited for

ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Storage

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4° C--at least 10 years.Rreconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days

Relevant references

no information yet

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.

Properties

If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Latin name

Capra aegagrus hircus

Additional isotype

IgM

Conjugate

Peroxidases with EC number 1.11.1.x are catalyzing the reaction of ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH

French translation

anticorps