Goat anti Bovine IgG (H + L) (biotin)

Name

Goat anti Bovine IgG (H + L) (biotin)

Size

1.5 ml

Catalog number

43R-IG027bt

Price

375 EUR

More details

Applications

ELISA, FC, ICC, IHC, WB

Concentration

NA

Product Subtype

Conjugated Secondary Antibodies

Research Area

Immunology

Product Type

Secondary Antibodies

Immunogen

Goat anti-bovine IgG (H + L) (biotin) was raised in goat using bovine IgG (H & L) as the immunogen.

Specificity

Bovine

Form & Buffer

Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3.

Storage

Store at 4 deg C.

Shipping Info

Blue Ice

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Conjugation

Biotinylated

Latin name

Capra aegagrus hircus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG