Protein A antibody FITC

Name

Protein A antibody FITC

Size

100 µl

Catalog number

60C-CF0601

Price

352 EUR

More details

Category

Primary Antibody

Antibody Subtype

Conjugated Polyclonal Antibodies

Area of research

Immunology

Type of Immunogen

Protein A antibody (FITC) was raised in goat using protein A from Staphylococcus aureus ..

Raised in

Goat

Specificity

Staphylococcus aureus protein A

Cross Reactivity

Should be determined by the end user

Conjugate/Tag

FITC

Method of Purification

Protein A antibody (FITC) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Form & Buffer

Liquid in 0.01M Sodium Phosphate, 0.14M NaCl, pH 7.4 with 10mg/ml BSA (IgG and Protease free) and 0.01% Thimerosol

Storage

Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.

Shipping conditions

Blue Ice

Tested for

IF; FC

Usage Recommendations

FC: 1:2,000-1:10,000, IF: 1:500-1:2,500

Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.

Conjugation

Anti-FITC Antibody

French translation

anticorps