IL1b antibody biotin

Name

IL1b antibody biotin

Size

50 µg

Catalog number

60R-IG152BT

Price

833 EUR

More details

Category

Primary Antibody

Antibody Subtype

Conjugated Polyclonal Antibodies

Area of research

Cytokines & Growth Factors

Type of Immunogen

IL1b antibody (biotin) was raised in goat using E. Coli derived recombinant human IL1 beta/IL1F2 ..

Raised in

Goat

Specificity

Humen IL1b

Cross Reactivity

Should be determined by the end user

Isotypes

IgG

Conjugate/Tag

Biotin

Form & Buffer

Lyophilized from sterile filtered PBS solution with BSA. Reconstitute to a concentration of 50 ug/ml with 1 ml of sterile 20 mM Trizma Base, 150 mM NaCl, pH 7.3 containing 0.1% BSA.

Storage

Store at -20 deg C until reconstitution. Following reconstitution product may be stored at 4 deg C in the short term. For long term storage aliquot and freeze at -20 deg C. Avoid repeated freeze/thaw cycles.

Shipping conditions

Blue Ice

Tested for

ELISA; IHC; WB

Usage Recommendations

ELISA: 2-8 ug/ml, IHC: 515 ug/ml, WB: 0.1 ug/ml

Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Conjugation

Biotinylated

French translation

anticorps