Goat anti Swine IgM FITC

Name

Goat anti Swine IgM FITC

Size

1 mg

Catalog number

43C-CB1416

Price

315 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Raised in

Goat

Specificity

Pig

Cross Reactivity

Antiserum was solid phase adsorbed to ensure class specificity

Isotypes

IgG

Conjugate/Tag

FITC

Method of Purification

Goat anti Swine IgM (FITC) was purified by affinity chromatography.

Form & Buffer

PBS with 0.2% BSA and 0.09% NaN3.

Storage

Store at 4 deg C.

Shipping conditions

Blue Ice

Tested for

ICC; IHC; IF

Usage Recommendations

ICC, IHC and IF: 1:50 - 1:500

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus

Additional isotype

IgM