Goat anti Rat IgG H + L FITC
1 mg
43C-CB1237
414 EUR
Secondary Antibody
Conjugated Secondary Antibodies
Immunology
Goat anti-rat IgG (H+L) (FITC) was raised in goat using rat IgG whole molecule ..
Goat
Rat IgG (H + L)
Should be determined by the end user
FITC
Goat anti Rat IgG (H + L) (FITC) was purified by affinity chromatography.
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Blue Ice
ELISA; FC; IF
ELISA: 1:10,000-1:50,000, FC: 1;500-1:2,500, IF: 1:1,000-1:5,000
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
Anti-FITC Antibody
Capra aegagrus hircus,Rattus norvegicus
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
IgG