Goat anti Mouse IgG1 Alexa Fluor 488

Name

Goat anti Mouse IgG1 Alexa Fluor 488

Size

500 µg

Catalog number

43R-1649

Price

537 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Raised in

Goat

Specificity

Mouse IgG1 Fc gamma specific

Cross Reactivity

Minimal cross reactivity with human, bovine and rabbit serum proteins. Does not react with other mouse IgG subtypes, IgM or the Fab portion of mouse IgG1.

Isotypes

IgG1

Conjugate/Tag

Alexa Fluor 488

Method of Purification

Goat anti Mouse IgG1 was purified by affinity chromatography

Concentration

1.4 mg/ml

Form & Buffer

Provided as a lyophilized powder in 0.01M sodium phosphate, 0.25 M NaCl, pH 7.6, and 0.05% NaN3, with 15 mg/ml BSA.

Storage

Store at 2-8 deg C

Shipping conditions

Blue Ice

Tested for

ELISA; Immunoassays

Usage Recommendations

Dilution Range of 1:100 - 1:800 for most applications

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Goat anti Mouse IgG1 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.

Conjugation

Alexa Fluor

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.

Latin name

Capra aegagrus hircus,Mus musculus

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.