Goat anti Mouse IgG FITC

Name

Goat anti Mouse IgG FITC

Size

2 mg

Catalog number

43R-1359

Price

204 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Purified Mouse IgG

Raised in

Goat

Specificity

This antibody reacts with heavy (gamma) chains on mouse IgG.

Cross Reactivity

No reactivity is observed to non-immunoglobulin human serum proteins or light chains on all mouse immunoglobulins or F(ab)’2 fragments of mouse IgG.

Conjugate/Tag

FITC

Grade & Purity

Grater than 95% pure

Method of Purification

Affinity purified using solid phase Mouse IgG (H&L)

Source

Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Concentration

1.0 mg/ml (lot specific)

Form & Buffer

Clear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.

Storage

Store at 2-8 deg C.

Shipping conditions

Blue Ice

Tested for

FC; IF; IHC

Usage Recommendations

The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.

Properties

This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.

Conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus,Mus musculus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.