Goat anti Monkey IgM biotin

Name

Goat anti Monkey IgM biotin

Size

1 mg

Catalog number

43R-IG070bt

Price

401 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Goat anti-monkey IgM (biotin) was raised in goat using monkey IgM ..

Raised in

Goat

Specificity

Monkey IgM

Cross Reactivity

Should be determined by the end user

Conjugate/Tag

Biotin

Method of Purification

Goat anti Monkey IgM (biotin) was purified by delipidation, selective precipitation and tandem molecular sieve chromatography followed by dialysis.

Form & Buffer

Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.

Storage

Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping conditions

Blue Ice

Tested for

ELISA; IHC; WB

Usage Recommendations

ELISA: 1:20,000-1:300,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Rhesus Monkeys proteins are often measured by ELISA on serum or plasma since consensus epitopes with the human ELISA are used for producing the antibodies of these ELISA test kits. Often cDNA of monkeys is used as alternative to human cDNA as a model for drug development. Monkeys are generally considered to be intelligent, particularly Old World monkeys.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Conjugation

Biotinylated

Latin name

Capra aegagrus hircus

Additional isotype

IgM