Goat anti Human IgM biotin

Name

Goat anti Human IgM biotin

Size

1 mg

Catalog number

43C-CB0997

Price

327 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Goat anti-human IgM (biotin) was raised in goat using human IgM mu chain ..

Raised in

Goat

Specificity

Human IgM

Cross Reactivity

Should be determined by the end user

Conjugate/Tag

Biotin

Method of Purification

Goat anti Human IgM (biotin) was purified by affinity chromatography.

Form & Buffer

Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0 01% NaN3. I mMunoglobulin and protease free.

Storage

Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping conditions

Blue Ice

Tested for

ELISA; IHC; WB

Usage Recommendations

ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Conjugation

Biotinylated

Latin name

Capra aegagrus hircus

Additional isotype

IgM