Goat anti Human IgG Fc biotin

Name

Goat anti Human IgG Fc biotin

Size

1 ml

Catalog number

43R-IG017bt

Price

290 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Goat anti-human IgG Fc (biotin) was raised in goat using human igG, Fc fragment ..

Raised in

Goat

Specificity

Human

Cross Reactivity

Should be determined by the end user

Conjugate/Tag

Biotin

Method of Purification

Goat anti Human IgG Fc (biotin) was purified by affinity chromatography.

Form & Buffer

Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3.

Storage

Store at 4 deg C.

Shipping conditions

Blue Ice

Tested for

ELISA; ICC; IHC; WB

Usage Recommendations

ELISA: 1:20,000-1:400,000, ICC: 1:500-1:5,000, IHC: 1:500-1:5,000, FC: 1:200-1:1,000, WB: 1:20,000-1:400,000

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Conjugation

Biotinylated

Latin name

Capra aegagrus hircus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG