Goat anti Human IgG Fc biotin
1 ml
43R-IG017bt
290 EUR
Secondary Antibody
Conjugated Secondary Antibodies
Immunology
Goat anti-human IgG Fc (biotin) was raised in goat using human igG, Fc fragment ..
Goat
Human
Should be determined by the end user
Biotin
Goat anti Human IgG Fc (biotin) was purified by affinity chromatography.
Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3.
Store at 4 deg C.
Blue Ice
ELISA; ICC; IHC; WB
ELISA: 1:20,000-1:400,000, ICC: 1:500-1:5,000, IHC: 1:500-1:5,000, FC: 1:200-1:1,000, WB: 1:20,000-1:400,000
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Biotinylated
Capra aegagrus hircus
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
IgG