Goat anti Chicken IgY H + L biotin

Name

Goat anti Chicken IgY H + L biotin

Size

500 µl

Catalog number

43R-1609

Price

303 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Goat anti-Chicken IgY (H + L) (biotin) was raised in Goat using purified chicken IgY (whole protein) emulsified in Freund’s adjuvant ..

Raised in

Goat

Specificity

Chicken IgY

Conjugate/Tag

Biotin

Method of Purification

Goat Anti-Chicken IgY (H + L) (biotin) was purified by affinity chromatography.

Concentration

1 mg/ml

Form & Buffer

Provided in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% NaN3 added as a preservative.

Storage

Store at 4 deg C for short term storage. For long term storage add glycerol, aliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles. Protect from light.

Shipping conditions

Blue Ice

Tested for

Please refer to the datasheet

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.Chicken yolk polyclonal Immunoglobulin Y (abbreviated as IgY) is a type of immunoglobulin which is the major antibody in bird, reptile, and lungfish blood. It is also found in high concentrations in chicken egg yolk. As with the other immunoglobulins, IgY is a class of proteins which are formed by the immune system in reaction to certain foreign substances, and specifically recognize them.

Conjugation

Biotinylated

Latin name

Capra aegagrus hircus