Goat anti Cat IgG biotin
2 mg
43C-CB0219
377 EUR
Secondary Antibody
Conjugated Secondary Antibodies
Immunology
Goat anti-cat IgG (biotin) was raised in goat using feline IgG F(c) fragment ..
Goat
Feline IgG
Should be determined by the end user
Biotin
Goat anti Cat IgG (biotin) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Blue Ice
ELISA; IHC; WB
ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Felix vulgaris genes or proteins used as antigen, synthetic peptide.
Biotinylated
Capra aegagrus hircus
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
IgG