FITC antibody

Name

FITC antibody

Size

1 mg

Catalog number

70R-FG001

Price

358 EUR

More details

Category

Primary Antibody

Antibody Subtype

Polyclonal Antibodies, Purified

Area of research

Miscellaneous

Type of Immunogen

FITC antibodies were raised in goat using fluorescein ..

Raised in

Goat

Cross Reactivity

Should be determined by the end user

Isotypes

IgG

Method of Purification

FITC antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Form & Buffer

Provided as a liquid in PBS, pH 7.2, with 0.1% NaN3.

Storage

Store at 4 deg C.

Shipping conditions

Blue Ice

Tested for

ELISA; ICC; IHC; WB

Usage Recommendations

ELISA: coating:1:100-1:500, primary:1:1,000-1:30,000, ICC: 1:200-1:2,000,IHC: 1:200-1:2,000, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000

Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.

Conjugation

Anti-FITC Antibody

French translation

anticorps