Biotin antibody
1 ml
20-BG20
240 EUR
Primary Antibody
Polyclonal Antibodies
Miscellaneous
Biotin antibodies were raised in goat using biotin conjugated to KLH ..
Goat
This serum reacts only to Biotin
Biotin antibody was purified by ion exchange chromatography followed by dialysis.
Monospecific antisera, absorbed, delipidated and defibrinated, Provided in 0.02M NaPO4, 0.15M NaCl, pH 7.2, with 0.01% NaN3
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Blue Ice
ELISA; IHC; WB
ELISA: 1:100,000-1:500,000, WB: 1:10,000-1:50,000
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Biotinylated
anticorps