Biotin antibody
1 mg
70R-BG003
229 EUR
Primary Antibody
Polyclonal Antibodies, Purified
Miscellaneous
Goat
Should be determined by the end user
IgG
Biotin antibody was purified by affinity chromatography.
1.0 mg/ml
Affinity purified and Provided in PBS, pH 7.2, with 0.1% NaN3.
Store at 4 deg C.
Blue Ice
ELISA; ICC; IHC; WB
ELISA: coating:1:100-1:500, primary:1:1,000-1:30,000, ICC: 1:200-1:2,000,IHC: 1:200-1:2,000, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Biotinylated
anticorps