alpha 1 Antitrypsin antibody biotin

Category

Primary Antibody

Antibody Subtype

Conjugated Polyclonal Antibodies

Area of research

Proteases, Inhibitors, & Enzymes

Type of Immunogen

alpha 1 Antitrypsin antibody (biotin) was raised in goat using human alpha-1-anti-Trypsin ..

Raised in

Goat

Specificity

Human alpha 1 Antitrypsin

Cross Reactivity

Conjugate/Tag

Biotin

Method of Purification

alpha 1 Antitrypsin antibody (biotin) was purified by ion exchange chromatography followed by dialysis.

Form & Buffer

Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.

Storage

Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.

Shipping conditions

Blue Ice

Tested for

ELISA; WB

Usage Recommendations

ELISA: 1:50,000, WB: 1:2,000-1:10,000

Description

The alpha 1 Antitrypsin antibody is a α- or alpha protein sometimes glycoprotein present in blood.

Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Conjugation

Biotinylated

French translation

anticorps