Rabbit anti Goat IgG H + L HRP

Name

Rabbit anti Goat IgG H + L HRP

Size

500 µg

Catalog number

43R-1076

Price

340 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Purified Goat IgG (H&L)

Raised in

Rabbit

Specificity

This antibody reacts with heavy chains on Goat IgG and light chains on all Goat immunoglobulins.

Cross Reactivity

No reactivity is observed to non-immunoglobulin Goat serum proteins IgG from human, mouse, or rat.

Conjugate/Tag

HRP

Grade & Purity

Grater than 90% pure

Method of Purification

Affinity purified using solid phase Goat IgG (H&L)

Source

Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Concentration

1.0 mg/ml (lot specific)

Form & Buffer

Lyophilized powder containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.1% (v/v) Kathon CG. Protease/IgG free, pH 7.2. Rehydrate with 0.55 ml of deionized water. Prepare fresh working dilution daily.

Storage

Store lyophilized material at 2-8 deg C.

Shipping conditions

Blue Ice

Tested for

Please refer to the datasheet

Usage Recommendations

1:200-1:5000 ELISA/WB, 1:500-1:5000 IHC

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Latin name

Capra aegagrus hircus,Oryctolagus cuniculus

Properties

HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

Conjugation

HRP Conjugated

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG