Goat anti Rat IgG H + L biotin
2 mg
43R-1534
327 EUR
Secondary Antibody
Conjugated Secondary Antibodies
Immunology
Purified Rat IgG (H&L)
Goat
This antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.
No reactivity is observed to non-immunoglobulin mouse serum proteins or IgG from human or mouse.
Biotin
Grater than 95% pure
Affinity purified using solid phase Rat IgG (H&L)
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
1.0 mg/ml (lot specific)
Clear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
Store at 2-8 deg C.
Blue Ice
Please refer to the datasheet
1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Biotinylated
Capra aegagrus hircus,Rattus norvegicus
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
IgG