Goat anti Mouse IgG H + L Alk Phos

Name

Goat anti Mouse IgG H + L Alk Phos

Size

1 mg

Catalog number

43R-1321

Price

290 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Purified Mouse IgG (H&L)

Raised in

Goat

Specificity

This antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins.

Cross Reactivity

No reactivity is observed to non-immunoglobulin mouse serum proteins or human IgG or serum proteins.

Conjugate/Tag

Alkaline Phosphatase

Grade & Purity

Grater than 95% pure

Method of Purification

Affinity purified using solid phase Mouse IgG (H&L)

Source

Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Concentration

1.50 mg/ml (lot specific)

Form & Buffer

Clear, colorless liquid, containing 30 mM Triethanolamine, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.

Storage

Store undiluted liquid at 2-8 deg C. For storage at -20 deg C, aliquot and dilute with an equal volume of glycerol to prevent loss of enzymatic activity.

Shipping conditions

Blue Ice

Tested for

Please refer to the datasheet

Usage Recommendations

1:5000-1:50,000 ELISA/WB, 1:500-1:2000 IHC

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.

Latin name

Capra aegagrus hircus,Mus musculus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.