Goat anti Human IgM mu chain FITC

Name

Goat anti Human IgM mu chain FITC

Size

500 µg

Catalog number

43R-1241

Price

327 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Purified Human IgM

Raised in

Goat

Specificity

This antibody reacts with heavy chains on human IgM (mu chain).

Cross Reactivity

No reactivity is observed to non-immunoglobulin human serum proteins light chains on all human immunoglobulins human IgA + IgG.

Conjugate/Tag

FITC

Grade & Purity

Grater than 90% pure

Method of Purification

Affinity purified using solid phase Human IgM

Source

Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Concentration

1.0 mg/ml (lot specific)

Form & Buffer

Clear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.

Storage

Store at 2-8 deg C.

Shipping conditions

Blue Ice

Tested for

FC; IF; IHC

Usage Recommendations

The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.

Properties

This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus

Additional isotype

IgM