Goat anti Human IgE epsilon chain biotin
1 mg
43R-1135
463 EUR
Secondary Antibody
Conjugated Secondary Antibodies
Immunology
Purified Human IgE
Goat
This antibody reacts with heavy chains on human IgE (epsilon chain).
No reactivity is observed to non-immunoglobulin human serum proteins or light chains on all human immunoglobulins or serum proteins from bovine, mouse or rabbit IgG1.
Biotin
Grater than 95% pure
Affinity purified using solid phase Human IgE
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
1.0 mg/ml (lot specific)
Clear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
Store at 2-8 deg C.
Blue Ice
Please refer to the datasheet
1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.Immunoglobulin E (IgE) kappa or Fc specific antibody is a kind of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is synthesized by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgEs play a role in allergy and response to parasite infection. High levels of IgEs caused by parasites can lower the allergic reaction of patients to allergens common present in the human environment. As such the parasite history of an allergic patient needs to be taken in consideration as a positive factor.
Biotinylated
Capra aegagrus hircus
IgE