Goat F(ab')2 Anti-Rabbit IgG (H+L), Mouse/Rat/Human ads-FITC

Name

Goat F(ab')2 Anti-Rabbit IgG (H+L), Mouse/Rat/Human ads-FITC

Size

500ug

Catalog number

MBS674770

Price

227 EUR

More details

Alternative name1

Anti-Goat F(ab')2 Anti-Rabbit IgG (H+L), Mouse/Rat/Human ads-FITC

Alternative name2

Caprine F(ab')2 Anti-Rabbit IgG (H+L), Murine/Rat/Human ads-FITC

Alternative name3

Caprinae F(ab')2 Anti-Lapin IgG (H+L), Mus musculus/Rattus norvegicus/Homo sapiens ads-FITC

Alternative name4

Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific)

Alternative name5

Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads - FITC; Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific) (Minimal cross reactivity with human, rat, & mouse immunoglobulins)

Other names

N/A

Gene name

N/A

Gene name synonims

N/A

Other gene names

N/A

Category

Secondary Antibodies

Clonality

Polyclonal

Immunoglobulin isotype

IgG

Clone

Polyclonal antibody

Host organism

Goat

Species reactivity

Rabbit

Specificity and cross-reactivity

Reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human, rat, and mouse immunoglobulins.

Purification method

Gel filtration of pepsin-digested antibody

Form/Appearance

Fluorescein (FITC) Conjugate

Concentration

N/A

Storage and shipping

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at The items should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at The items should be kept in the range of 1-7 degrees Celsius.. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at The items should be kept in the range of 1-7 degrees Celsius. or long-term productone should be stored at -20 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at The items should be kept in the range of 1-7 degrees Celsius..

Tested for:

ELISA; Immunoblot; Immunohistochemistry

Description

productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.

Advisory

In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Additional description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Group

f(ab')2s

Additional isotype

F(ab')2 Fragment Affinity-Purified Secondary Antibodies,IgG

Properties

This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus,Mus musculus,Oryctolagus cuniculus,Rattus norvegicus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by MBS Polyclonals. Rabbit antibodies are very stable and can be stored for several days at room temperature. MBS Polyclonals adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.