GOAT F(ab')2 ANTI RAT IgG:FITC (MOUSE ADSORBED) Antibody
500ul
MBS235148
343 EUR
Anti-GOAT F(ab')2 ANTI RAT IgG:FITC (MOUSE ADSORBED)
Caprine F(ab')2 ANTI RAT IgG:FITC (Murine ADSORBED)
Caprinae F(ab')2 ANTI Rattus norvegicus IgG:FITC (Mus musculus ADSORBED)
IgG
N/A
Ig gamma-2A chain C region; Ig gamma-2A chain C region; N/A; N/A; N/A
IgG
N/A
LOC679045; Igg-2a; N/A; N/A
Antibodies
Polyclonal
Polyclonal IgG
Polyclonal antibody
Goat
Rat
IgG This item recognises rat IgG. Cross-reactivity with mouse IgG has been reduced by adsorption, however, we recommend dilution in buffer containing 10% (v/v) normal mouse serum for use upon mouse tissues.
N/A
FITC (F(ab')2 fragment of IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
F(ab')2 concentration 0.7 mg/ml
Store at +Store productone at +4 degrees Celsius. or productone should be stored at -20 degrees Celsius. if preferred. productone should be stored undiluted. Storage in frost-free freezers is not recommended. productone is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should productone contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of dispatch.
Flow Cytometry (FACS)
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes.
f(ab')2s
F(ab')2 Fragment Affinity-Purified Secondary Antibodies,IgG
Anti-FITC Antibody
Capra aegagrus hircus,Mus musculus,Rattus norvegicus
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
anticorps