Goat anti-rabbit Ig (Secondary Ab)-FITC
200μg
GRIG-BSS200H
382 EUR
Antibody
Polyclonal
FITC
1ml
anti Rabbit
RUO
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
This AcZon Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. AcZon FITC is highly sensitive to pH extremes.
Anti-FITC Antibody
Capra aegagrus hircus,Oryctolagus cuniculus
Rabbits are used for polyclonal antibody production by AcZon. Rabbit antibodies are very stable and can be stored for several days at room temperature. AcZon adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.