Goat Anti-Mouse IgG (H&L) FITC

Name

Goat Anti-Mouse IgG (H&L) FITC

Size

100 µl

Catalog number

6919-100

Price

345 EUR

More details

Antibody Target

Mouse IgG

Target Alternative Name

Anti-mouse IgG, FITC-Linked Antibody, FITC linked secondary antibody

Category

Secondary Antibody

Antibody Type

Secondary antibody

Species Reactivity

Goat

Immunogen Sequence

Mouse IgG (H&L)

Accession

N/A

Gene ID

N/A

Appearance

Colorless liquid

Antibody Form

Liquid

Concentration

N/A

Formulation

In 0.01M Sodium phosphate, 0.25 M NaCl, 1% BSA, 50% Glycerol, pH 7.6 and 0.05% Sodium Azide.

Purification

Affinity Purified

Application

IF, FACS

Storage Temp.

-20 °C

Shipping

gel pack

Shelf Life

6 months

Usage

For Research Use Only! Not to be used in humans.

Handling

The antibody solution should be gently mixed before use.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.

Conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus,Mus musculus

Additional isotype

Heavy & Light chains,IgG

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.