Goat anti Rat IgG1 (subclass specific), conjugated with Horseradish peroxidase, Secondary Antibodies

Name

Goat anti Rat IgG1 (subclass specific), conjugated with Horseradish peroxidase, Secondary Antibodies

Size

1ml

Catalog number

GARa-IgG1-PO

Price

612 EUR

More details

Host/Source

Goat

Clone Name

Polyclonal

Product Category

Veterinary

Species Reactivity

Rat

Application

ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting

Immunogen

Pools of purified homogenous IgG1 isolated from pooled rat serum. FreundÍs complete adjuvant is used in the first step of the immunization procedure.

Purification Method

NA

Shipping Conditions

NA

Storage conditions

Goat anti Rat IgG1 (subclass specific), conjugated with Horseradish peroxidase, Secondary Antibodies philized conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days

Datasheet link

NA

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Latin name

Capra aegagrus hircus,Rattus norvegicus

Additional isotype

IgG1

Conjugate

Peroxidases with EC number 1.11.1.x are catalyzing the reaction of ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.