Goat Anti-Mouse IgG(H+L) Alexa Fluor 488-conjugated

Name

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488-conjugated

Size

200µL

Catalog number

S0017

Price

500 EUR

More details

Type

secondary antibody

Shipping

Ice bag

Antigen

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488-conjugated

Reactivity

Mouse

Clonality

Polyclonal

Application

WB,IF/ICC,FCM

Application_advice

IF/ICC 1:100 – 1:500

Conjugate

Alexa Fluor 488

Immunogen

The antiserum was developed in goat using the MOUSE IgG as the immunogen.

Purification

affinity purification.

Format

Liquid

Concentration

1mg/ml

Fragment

Fab fragment

Storage_Buffer

0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6._x000D_ Store at -20℃,avoid repeated thawing and freezing.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Goat Anti-Mouse IgG(H+L) -conjugated has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.

Conjugation

Alexa Fluor

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Latin name

Capra aegagrus hircus,Mus musculus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.