Swine anti Goat secretory IgA

Name

Swine anti Goat secretory IgA

Size

1 mL

Catalog number

SwAG/sIgA

Price

209 EUR

More details

Category

Secondary Antibodies

Long description

The reactivity of the antiserum is restricted to the Fc part of the IgA molecule and to surface and hidden determinants of the secretory component as tested in immunoelectrophoresis and radial immunodiffusion. In immunoelectrophoresis using various antiserum concentrations against normal goat milk two precipitin lines may be obtained, one representing IgA and the other the free secretory component. Against normal goat serum only IgA is precipitated. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in goat serum, milk or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of goat IgA from serum or exocrine secretions. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude such use if proper controls are include.

Antibody come from

Highly purified secretory IgA isolated from pooled goat milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Other description

Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal swine serum. No foreign proteins added.

Clone

Polyclonal

Antigen-antibody binding interaction

Swine anti Goat secretory IgA Antibody

Antibody is raised in

Swine

Antibody's reacts with

Caprine

Antibody's reacts with these species

The antiSerum does not cross-react with any other component of the Goat Ig system. Interspecies cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Antibody's specificity

Precipitating polyclonal Swine antiSerum to Goat secretory IgA.

Research interest

Veterinary

Application

Immunoprecipitation

Antibody's suited for

Immunoprecipitation. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Precipitin titre 1:32 when tested against pooled normal goat serum in agar-block immunodiffusion titration.

Storage

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days.

Relevant references

no information yet

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Latin name

Capra aegagrus hircus

Properties

Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs.

Additional isotype

IgA