Goat anti Monkey IgM (Fc specific) antibody conjugated with TRITC

Name

Goat anti Monkey IgM (Fc specific) antibody conjugated with TRITC

Size

1 mL

Catalog number

GAMon/IgM(Fc)/TRITC

Price

306 EUR

More details

Category

Secondary Antibodies

Long description

The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Direct immunofluorescence staining of cytoplasmic IgM of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.

Antibody come from

Purified polyclonal IgM and pools of homogenous IgM isolated from pooled monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Other description

TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Clone

Polyclonal

Antigen-antibody binding interaction

Goat anti Monkey IgM (Fc specific) antibody conjugated with TRITC Antibody

Antibody is raised in

Goat

Antibody's reacts with

Monkey

Antibody's reacts with these species

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with immunoglobulins in Serum of other old-world Monkeys, including Cercopithecus, Cynomolgus and Baboon. The product may also react with other species as has been observed for Chimpanzee and man.

Antibody's specificity

Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Monkey IgM, Fc specific.

Research interest

Veterinary

Application

ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence

Antibody's suited for

ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Storage

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days

Relevant references

no information yet

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Rhesus Monkeys proteins are often measured by ELISA on serum or plasma since consensus epitopes with the human ELISA are used for producing the antibodies of these ELISA test kits. Often cDNA of monkeys is used as alternative to human cDNA as a model for drug development. Monkeys are generally considered to be intelligent, particularly Old World monkeys.

Properties

If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Latin name

Capra aegagrus hircus

Additional isotype

IgM

French translation

anticorps