Pyruvate Kinase antibody IgG fraction

Name

Pyruvate Kinase antibody IgG fraction

Size

1 mg

Catalog number

20R-PG010

Price

167 EUR

More details

Category

Primary Antibody

Antibody Subtype

Polyclonal Antibodies

Area of research

Proteases, Inhibitors, & Enzymes

Type of Immunogen

Pyruvate kinase antibody (IgG fraction) was raised in goat using pyruvate kinase isolated from rabbit Muscle ..

Raised in

Goat

Specificity

Rabbit pyruvate kinase

Cross Reactivity

Should be determined by the end user

Method of Purification

Pyruvate Kinase antibody (IgG fraction) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Form & Buffer

Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.

Storage

Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.

Shipping conditions

Blue Ice

Tested for

ELISA; IHC; WB

Usage Recommendations

ELISA: 1:4,000-1:20,000, IHC: 1:500-1:2,000, WB: 1:500-1:2,000

Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG

French translation

anticorps