Protein A antibody biotin
10 mg
60C-CF0603
672 EUR
Primary Antibody
Conjugated Polyclonal Antibodies
Immunology
Protein A antibody (biotin) was raised in goat using Protein A [Staphylococcus aureus] ..
Goat
Staphylococcus aureus protein A
Should be determined by the end user
Biotin
Protein A antibody (biotin) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
IgG fraction Provided as a liquid in 20 mM Potassium Phosphate and 150 mM NaCl, pH 7.2, with 10mg/ml BSA (IgG and Protease free) as a stabilizer, and 0.01% NaN3.
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Blue Ice
ELISA; IHC; WB
ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Biotinylated
anticorps