Goat anti Rat IgM FITC

Name

Goat anti Rat IgM FITC

Size

1 mg

Catalog number

43C-CB1272

Price

389 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Goat anti-rat IgM (FITC) was raised in goat using rat IgM mu chain ..

Raised in

Goat

Cross Reactivity

Should be determined by the end user

Conjugate/Tag

FITC

Method of Purification

Goat anti Rat IgM (FITC) was purified by delipidation, selective precipitation and tandem molecular sieve chromatography followed by dialysis.

Form & Buffer

Lyophilized. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 10 mg/ml Bovine Serum Albumin (BSA)-I mMunoglobulin and Protease free. 0.01% (w/v) NaN3

Storage

Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping conditions

Blue Ice

Tested for

ELISA; FC; IF

Usage Recommendations

ELISA: 1:10,000-1:50,000, FC: 1;500-1:2,500, IF: 1:1,000-1:5,000

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus,Rattus norvegicus

Additional isotype

IgM

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.