Goat anti Rat IgG H + L HRP

Name

Goat anti Rat IgG H + L HRP

Size

2 mg

Catalog number

43C-CB1227

Price

352 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Goat anti-rat IgG (H+L) (HRP) was raised in goat using rat IgG whole molecule ..

Raised in

Goat

Specificity

Rat IgG (H + L)

Cross Reactivity

Should be determined by the end user

Conjugate/Tag

HRP

Method of Purification

Goat anti Rat IgG (H + L) (HRP) was purified by affinity chromatography.

Form & Buffer

Lyophilized. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 10 mg/ml Bovine Serum Albumin (BSA)-I mMunoglobulin and Protease free. 0.01% (w/v) Gentamicin Sulfate (Do NOT add NaN3!)

Storage

Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping conditions

Blue Ice

Tested for

ELISA; IHC; WB

Usage Recommendations

ELISA: 1:50,000-1:250,000, IHC: 1:1,000-1:5,000, WB: 1:5,000-1:25,000

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Latin name

Capra aegagrus hircus,Rattus norvegicus

Properties

HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

Conjugation

HRP Conjugated

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Additional isotype

IgG